Contact an Account ManagerImproved and simplified tissue extraction method for quantitating long-chain acyl-coenzyme A thioesters with picomolar detection using HPLC
Summary
Type: HPLC
Application #: 100290
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: UV 254nm
Conditions: eluent A: acetonitrile,
eluent B: 10mM KH2PO4 buffer pH 5.3,
linear gradient from 24 to 34% A in 4min, to 45% A in 10min, to 57% A in 9min, to 70% A in 8min,
flow rate: 1ml/min.
Authors: Mangino M J
Source: J Chromatogr 577 (1992) 157 - 162
Details
Legend: Analysis of long-chain acyl-CoA esters from canine renal cortex:
myristoyl-CoA,
palmitoleoyl-CoA,
linoleoyl-CoA,
arachidonyl-CoA,
palmitoyl-CoA,
oleoyl-CoA,
heptadecanoyl-CoA (int. std.)